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CompTech B105 说明书

 更新时间:2018-07-04 点击量:1330

 

上海起发为complementtech一级经销商,Complement Technology,Inc.于2005年成立.CompTech于2005年收购Advanced Research TechnologiesComplement Technology继续为世界各地的科学家提供高质量的试剂,并在我们的清单中增加了许多新试剂。我们尽力为您的应用提供试剂和使用我们补充试剂的建议。 

CompTech  B105  说明书

品名:     GVBE (GVBo with EDTA)  

货号:     B105         和    B104

规格 1000 mL和 250 mL              

成分:              0.1%明胶,5 mM Veronal,145 mM NaCl,0.025%NaN 3

 10mM EDTA,pH 7.3。

形式:       液体

缓冲液:  乙烯            钠

防腐剂:    0.025%sodium azide  作为杀菌剂进行无菌过滤

储存:      + 4 o C    避免冻结,导致明胶凝胶化。  如果冷冻,加热重新 溶解明胶。

预防措施:叠氮化 物对所有生物都有毒。              

产地:                        制造于美国。

 

一般描述

            GVBE是用于补体测定的等渗缓冲液,用于在孵育结束时添加时防止补体激活或停止正在进行的补体激活(Morgan,BP(2000); Dodds,AW和Sim,RB(1997))。

 

 

缓冲组件

            在该缓冲液中使用EDTA来螯合钙和镁,从而通过所有三种途径抑制补体活化。Veronal用作缓冲液,因为在20世纪中期,这是pH范围7.2-7.4的适合缓冲液,其不螯合金属离子并且不像其他缓冲液那样抑制补体反应。存在氯化钠以提供等渗环境,使得细胞不会由于渗透压而溶解。存在明胶以防止由于在稀释期间和在测定本身中吸附到或管上而导致的蛋白质组分的损失。 存在叠氮化物以防止细菌生长。       

 

物理特征

            该缓冲液中明胶的浓度低于形成固体凝胶的浓度。  然而,由于明胶是接近其胶凝浓度在4 ö在该温度下静置C期间明胶形式的一些字符串。  它们可以 通过加热到37 o C或在微波炉中短暂加热来轻松再溶解。

 

应用

            GVBE通常用作在测定结束时添加的缓冲液以停止补体活化并将反应稀释至可通过分光光度计读取的水平。  它还用于建立不应发生补体激活的对照试验(背景对照试验)。

           

参考

摩根,BP编辑。(2000)补充方法和协议。  Humana新闻。

 

Dodds,AW和Sim,RB编辑(1997年)。 补充实用方法(ISBN 019963539)牛津 大学 按, 牛津。

 

 

 

 

 

 

complementtech产品目录:

 

货号

品名

规格 

目录价

品牌

A098

C1

200µg

3400

complementtech

A099

C1q

1000µg

3162

complementtech

A100

C1q (40% Glycerol)

1000µg

3162

complementtech

A102

C1r Enzyme

250µg

3417

complementtech

A103

C1s Proenzyme

250µg

3366

complementtech

A104

C1s Enzyme

250µg

3281

complementtech

A140

C1 Esterase Inhibitor

1000µg

3468

complementtech

A112

C2

50µg

3298

complementtech

A113

C3

250µg

2482

complementtech

A113c

C3 (concentrated)

1000µg

7327

complementtech

A118

C3a

50µg

3162

complementtech

A119

C3a desArg

50µg

3043

complementtech

A114

C3b

250µg

3094

complementtech

A115

iC3b

250µg

3417

complementtech

A116

C3c

250µg

3706

complementtech

A117

C3d

100µg

3825

complementtech

A105

C4

250µg

3468

complementtech

A105c

C4 (concentrated)

1000µg

8245

complementtech

A106

C4a

50µg

4114

complementtech

A107

C4a desArg

50µg

4114

complementtech

A108

C4b

250µg

2992

complementtech

A109

C4b Binding Protein

250µg

4131

complementtech

A120

C5

250µg

3570

complementtech

A144

C

30µg

6188

complementtech

A144(300)

C

300µg

询价

complementtech

A145

C desArg

50µg

4301

complementtech

A122

C5b,6

50µg

3468

complementtech

A123

C6

250µg

3366

complementtech

A124

C7

250µg

3366

complementtech

A125

C8

250µg

3536

complementtech

A126

C9

250µg

3366

complementtech

A127

SC5b-9 Complex

100µg

2992

complementtech

A135

Factor B

250µg

3213

complementtech

A136

Factor D

25µg

3417

complementtech

A137

Factor H

250µg

2958

complementtech

A137c

Factor H (conc.)

10000µg

询价

complementtech

A138

Factor I

100µg

3366

complementtech

A139

Factor P

100µg

3468

complementtech

A150

Cobra Venom Factor  (USA ONLY)

1000µg

3298

complementtech

A154

Factor Ba

100µg

3298

complementtech

A155

Factor Bb

250µg

6188

complementtech

A118

C3a

50µg

3162

complementtech

A119

C3a desArg

50µg

3043

complementtech

A106

C4a

50µg

4114

complementtech

A107

C4a desArg

50µg

4114

complementtech

A144

C

30µg

6188

complementtech

A144(300)

C

300µg

询价

complementtech

A145

C desArg

50µg

4301

complementtech

A300

C1q-Dpl

1ml

2074

complementtech

A312

C2-Dpl

1ml

2074

complementtech

A314

C3-Dpl

1ml

2482

complementtech

A305

C4-Deficient Guinea Pig

1ml

2448

complementtech

A308

C4-Dpl           

1ml

2482

complementtech

A320

C5-Dpl

1ml

2125

complementtech

A323

C6-Dpl

1ml

2040

complementtech

A324

C7-Dpl

1ml

2074

complementtech

A325

C8-Dpl

1ml

2074

complementtech

A326

C9-Dpl

1ml

2074

complementtech

A335

Factor B-Dpl

1ml

2074

complementtech

A336

Factor D-Dpl   

1ml

2074

complementtech

A337

Factor H-Dpl

1ml

2686

complementtech

A338

Factor I-Dpl    

1ml

2482

complementtech

A339

Factor P-Dpl

1ml

2482

complementtech

A340

C3/C4 Inactivated Serum

1ml

2074

complementtech

A399

C1q/Factor D-Dpl  

1ml

2856

complementtech

A200

Goat Anti-Human C1q     

1ml

1054

complementtech

A212

Goat Anti-Human C2

1ml

2329

complementtech

A213

Goat Anti-Human C3

1ml

1054

complementtech

A218

Rabbit Anti-Human C3a

1ml

2550

complementtech

A205

Goat Anti-Human C4

1ml

1054

complementtech

A206

Rabbit Anti-Human C4a

1ml

2550

complementtech

A220

Goat Anti-Human C5

1ml

1275

complementtech

A221

Rabbit Anti-Human rC

1ml

2550

complementtech

A223

Goat Anti-Human C6

1ml

1275

complementtech

A224

Goat Anti-Human C7

1ml

1275

complementtech

A225

Goat Anti-Human C8

1ml

1275

complementtech

A226

Goat Anti-Human C9

1ml

1275

complementtech

A227

Rabbit Anti-Human SC5b-9 Neo (lgG)

0.2ml

2584

complementtech

A235

Goat Anti-Human Factor B

1ml

1275

complementtech

A236

Goat Anti-Human Factor D

1ml

2499

complementtech

A237

Goat Anti-Human Factor H

1ml

1275

complementtech

A238

Goat Anti-Human Factor I

1ml

1309

complementtech

A239

Goat Anti-Human Factor P

1ml

1462

complementtech

A240

Goat Anti-Human C1-INH        

1ml

1173

complementtech

A260

Rabbit Anti-Human Vitronectin

1ml

1445

complementtech

NHS

Human Serum

1ml

578

complementtech

GPS

Guinea Pig Serum

1ml

714

complementtech

NRS

Rat Serum

1ml

901

complementtech

NMS

Mouse Serum   

1ml

1309

complementtech

B102

GVB++ (with Ca and Mg)

250ml

1224

complementtech

B100

GVB++ (with Ca and Mg)

1 Liter

3264

complementtech

B103

GVB° (without Ca and Mg)

250ml

1224

complementtech

B101

GVB° (without Ca and Mg)

1 Liter

3264

complementtech

B104

GVBE (with EDTA)

250ml

1224

complementtech

B105

GVBE (with EDTA)

1 Liter

3264

complementtech

B110

SGVB++(low ionic strength w/Sucrose)

250ml

1870

complementtech

B111

SGVB++(low ionic strength w/Sucrose)

1 Liter

5066

complementtech

B112

VBS++ (with Ca and Mg)

250ml

1224

complementtech

B113

VBS++ (with Ca and Mg)

1 Liter

3264

complementtech

B200

EA (Ab-sensitized sheep E)

5ml @ 5x108/ml

3043

complementtech

B201

EA (Ab-sensitized sheep E)

10ml @ 5x108/ml

4556

complementtech

B202

EA (Ab-sensitized sheep E)

20ml @ 5x108/ml

6324

complementtech

B210

Es (sheep E)

20ml @ 5x108/ml

2652

complementtech

B300

Er (rabbit E)

5ml @ 5x108/ml

1649

complementtech

B301

Er (rabbit E)

10ml @ 5x108/ml

2652

complementtech

B302

Er (rabbit E)

20ml @ 5x108/ml

3893

complementtech

B400

Zymosan (preactivated)

10ml @ 1x109/ml

2261

complementtech

B106

MgEGTA

10ml @ 0.1M

1139

complementtech

B250

MCAR

1.0 ml

2482

complementtech

Hemolysin

Hemolysin  NEW

2.0 ml

2040

complementtech

 

 

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