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Cyandye Lot 10002说明书

 更新时间:2018-06-21 点击量:1284

上海起发为Cyandye特约经销商,Cyandye是一家以合理的价格向处理分子生物学的科学家提供化学服务的公司。Cyandye的专长是生物技术和生命科学的荧光染料。

 

Cyandye  Lot 10002说明书:

 

 

货号

品名

规格

品牌

 Lot 10002

Azidobutyrate NHS Ester

50 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

100 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

250 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

50 mg

cyandye

 

 

分子式:C 8 H 10 N 4 O 4分子量
:226.19 
同义词:

  • 1 - [(4- azidobutanoyl)氧基]吡咯烷-2,5-二酮
  • 琥珀酰亚胺4-叠氮丁酸酯
  • NHS-C3叠氮
  • 叠氮丁酸氧基琥珀酰亚胺酯>

 

对于标记0.2微摩尔的氨基修饰的寡核苷酸,在60μL的ACN中溶解2.3mg的叠氮丁酸NHS酯(DMSO或DMF也是可接受的)。将氨基修饰的寡核苷酸溶解在500μL的0.1M碳酸盐/碳酸氢盐缓冲液(pH 9)中。加入12μL的Azidobutyrate NHS酯溶液到溶解的寡核苷酸中,并在室温下反应2小时并脱盐。

储存:干燥,-10至-30°C 
稀释剂:无水ACN,DMSO或DMF溶液
稳定性:2-3天

质量控制: NMR:95%TLC:纯

AZIDOBUTYRATE使用

在这种情况下,在任何合成器上像往常一样合成寡核苷酸。氨基必须在寡核苷酸的3'或5'端引入。在裂解和脱保护之后,叠氮基可以作为胺的修饰被引入。例如,碳酸钠/碳酸氢钠缓冲液(pH8.75)中的寡核苷酸在室温下与琥珀酰亚胺基-4-叠氮基丁酸酯在DMSO中孵育。然后使用HPLC纯化终的寡核苷酸或用乙醇或丁醇沉淀。

参考文献:
J. Am。化学。
使用Click Chemistry
Langmuir,2010,26(11),pp 8666-8672 DOI:10.1021 / ja070273v Template-Directed Oligonucleotide Strand Ligation,Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry Langmuir,2010,26(11),pp 8666-8672 DOI: 10.1021 / la904822f 
将脂质囊泡共价连接到液体支持的双层允许观察DNA介导的囊泡相互作用

 

 

 

Cyandye 产品列表:

 

货号

品名

规格

品牌

 Lot 10002

Azidobutyrate NHS Ester

50 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

100 mg

cyandye

 Lot 10002

Azidobutyrate NHS Ester

250 mg

cyandye

 Lot 10007

Cy3 carboxylic acid

5 mg

cyandye

 Lot 10007

Cy3 carboxylic acid

100 mg

cyandye

Lot 10006

Cy5 carboxylic acid

5 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

5 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

25 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

50 mg

cyandye

Lot 10012

Cy5.5 carboxylic acid

100 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

1 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

5 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

10 mg

cyandye

 Lot 10013

diSulfo - Cy3 alkyne

25 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

1 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

5 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

10 mg

cyandye

Lot 10022

diSulfo - Cy3 azide

25 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

5 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

25 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

50 mg

cyandye

Lot a0015

diSulfo - Cy3 carboxylic acid

100 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

5 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

25 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

50 mg

cyandye

Lot 10017

diSulfo - Cy3 NHS Ester

100 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

1 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

5 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

10 mg

cyandye

Lot 10016

diSulfo - Cy5 alkyne

25 mg

cyandye

 Lot 10023

diSulfo - Cy5 azide

1 mg

cyandye

 Lot 10023

diSulfo - Cy5 azide

5 mg

cyandye

 Lot 10010

diSulfo - Cy5 carboxylic acid

5 mg

cyandye

 Lot 10010

diSulfo - Cy5 carboxylic acid

100 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

5 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

25 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

50 mg

cyandye

Lot 10009

diSulfo - Cy5 Hydrazide

100 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

5 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

25 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

50 mg

cyandye

Lot 10014

diSulfo - Cy5 NHS Ester

100 mg

cyandye

Lot 10027

diSulfo - Cy7 carboxylic acid

5 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

5 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

25 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

50 mg

cyandye

Lot a0021

diSulfo - Cy7 NHS Ester

100 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

1 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

5 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

10 mg

cyandye

Lot 10004

monoSulfo - Cy3 alkyne

100 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

1 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

5 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

10 mg

cyandye

Lot 10011

monoSulfo - Cy3 azide

100 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

5 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

25 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

50 mg

cyandye

Lot 10025

monoSulfo - Cy3 carboxylic acid

100 mg

cyandye

Lot 10008

monoSulfo - Cy3 NHS Ester

5 mg

cyandye

Lot 10008

monoSulfo - Cy3 NHS Ester

100 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

1 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

5 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

10 mg

cyandye

Lot 10020

monoSulfo - Cy5 azide

100 mg

cyandye

 Lot 10026

monoSulfo - Cy5 carboxylic acid

5 mg

cyandye

 Lot 10026

monoSulfo - Cy5 carboxylic acid

25 mg

cyandye

 Lot 10018

monoSulfo - Cy5 NHS Ester

5 mg

cyandye

 Lot 10018

monoSulfo - Cy5 NHS Ester

25 mg

cyandye

Lot a0024

3-azidopropylamine

100 mg

cyandye

 Lot d2501

2'-Deoxy-5'-acetyl-5-acetoxymethyluridine

250 mg

cyandye

 Lot a0001

2'-Deoxy-5-hydroxymethyluridine

250 mg

cyandye

 Lot a0001

2'-Deoxy-5-hydroxymethyluridine

500 mg

cyandye

Lot a0029

4-hydrazinobenzene-1,3-disulfonic acid

500 mg

cyandye

Lot a0028

5-(hydroxymethyl)-1-[(6aR,8R,9aS)-2,2,4,4-tetraisopropyltetrahydro-6H-furo[3,2-f][1,3,5,2,4]trioxadisilocin-8-yl]pyrimidine-2,4(1H,3H)-dione

50 mg

cyandye

Lot a0019

2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-ol

500 mg

cyandye

Lot a0003

2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose

50 mg

cyandye

Lot a0003

2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose

100 mg

cyandye

Lot a0003

2,3,4,6-tetra-O-benzoyl-1-O-(2,2,2-trichloroethanimidoyl)-α-D-glucopyranose

250 mg

cyandye

 

 

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